Ohio State is in the process of revising websites and program materials to accurately reflect compliance with the law. While this work occurs, language referencing protected class status or other activities prohibited by Ohio Senate Bill 1 may still appear in some places. However, all programs and activities are being administered in compliance with federal and state law.

Metabarcoding Pipeline

Metabarcoding Workflow

metabarcoding pipeline see text below

Metabarcoding Workflow (text version)

  1. Read Processing (DADA2)
    • Filtering and trimming
    • Error learning
    • Dereplication and denoising
    • Amplicon merging
    • Chimera removal
  2. Amplicon Sequence Variant (ASV) identification (dada2)
    • Taxonomic assignment (SILVA, but other possible)
    • Abundance quantification
  3. ASV analyses (Rmarkdown)
    • Contaminant removal (phyloseq + decontam)
    • Alpha diversity (vegan)
    • Beta diversity (vegan)
    • Composition and most abundant members (tidyverse)
    • Differential abundance (ANCOM-BC)