2021 Viromics Workshop Webinar Series

2021 Viromics Workshop Webinar Series

Foreword

As the microbiome has gained increasing attention across ecosystems ranging from oceans and soils to humans and bioreactors, so too have the viruses that infect these microbiota.


Since 2013, we have run in-person training workshops in viral ecology, with viromics taking center stage since 2016.


This has introduced hundreds of trainees to the challenges and opportunities that come with studying viruses in complex communities, and gave rise to ‘iVirus’, our virus ecogenomics platform on NSF’s CyVerse Cyberinfrastructure.


Different to past workshops, this one focuses less on an overview of viral ecogenomics and instead on a deep dive of several timely topics where we will hear from the experts building the tools, and then open the floor for ‘discussion’ — all held virtually to make as broadly accessible as possible.


Enjoy!

– Matt Sullivan

Registration

Registration has now closed.

Social Media

Please share this event in your network and use #viromics_workshop2021 for all Twitter mentions during the workshop.

Twitter handles for most speakers are in their bio, below.

Contact

For technical issues and/or general questions, please contact:

  • Olivier Zablocki (zablocki.4@osu.edu or DM @OlivierZablo)
  • Brittany Fonner (fonner.11@osu.edu)

Organizing Committee

  • Evelien Adriaenssens: Quadram Institute, UK
  • Ben Bolduc: Ohio State University, USA
  • Joanne Emerson: University of California, Davis, USA
  • Manja Marz: Friedrich Schiller University Jena, Germany
  • Simon Roux: Joint Genome Institute, USA
  • Matt Sullivan: Ohio State University, USA
  • Ben Temperton: University of Exeter, UK
  • Arvind Varsani: Arizona State University, USA

Coordination & Logistics

Olivier Zablocki, Ohio State University, USA
Brittany Fonner, Ohio State University, USA

Should you have any questions or experience any issues during the webinar, please contact either Olivier (zablocki.4@osu.edu) or Brittany (fonner.11@osu.edu)

Suggested Reading

  • Adriaenssens, E., & Brister, J. R. (2017). How to name and classify your phage: an informal guide. Viruses9(4), 70. LINK
     
  • Turner, D., Kropinski, A. M., & Adriaenssens, E. M. (2021). A roadmap for genome-based phage taxonomy. Viruses13(3), 506. LINK
     
  • Kieft, K., Zhou, Z., & Anantharaman, K. (2020). VIBRANT: automated recovery, annotation and curation of microbial viruses, and evaluation of viral community function from genomic sequences. Microbiome8(1), 1-23. LINK
     
  • Jang, H. B., Bolduc, B., Zablocki, O., Kuhn, J. H., Roux, S., Adriaenssens, E. M., … & Sullivan, M. B. (2019). Taxonomic assignment of uncultivated prokaryotic virus genomes is enabled by gene-sharing networks. Nature biotechnology37(6), 632-639. LINK
     
  • Bolduc, B., Jang, H. B., Doulcier, G., You, Z. Q., Roux, S., & Sullivan, M. B. (2017). vConTACT: an iVirus tool to classify double-stranded DNA viruses that infect Archaea and Bacteria. PeerJ5, e3243. LINK
     
  • Mitchell, A. L., Almeida, A., Beracochea, M., Boland, M., Burgin, J., Cochrane, G., … & Finn, R. D. (2020). MGnify: the microbiome analysis resource in 2020. Nucleic acids research48(D1), D570-D578. LINK
     
  • Moraru, C., Varsani, A., & Kropinski, A. M. (2020). VIRIDIC—A novel tool to calculate the intergenomic similarities of prokaryote-infecting viruses. Viruses12(11), 1268. LINK
     
  • Moraru, C. (2021). VirClust–a tool for hierarchical clustering, core gene detection and annotation of (prokaryotic) viruses. bioRxiv. LINK
     
  • Pons, J. C., Paez-Espino, D., Riera, G., Ivanova, N., Kyrpides, N. C., & Llabrés, M. (2021). VPF-Class: taxonomic assignment and host prediction of uncultivated viruses based on viral protein families. Bioinformatics. LINK
     
  • Roux, S., Enault, F., Hurwitz, B. L., & Sullivan, M. B. (2015). VirSorter: mining viral signal from microbial genomic data. PeerJ3, e985. LINK
     
  • Guo, J., Bolduc, B., Zayed, A. A., Varsani, A., Dominguez-Huerta, G., Delmont, T. O., … & Roux, S. (2021). VirSorter2: a multi-classifier, expert-guided approach to detect diverse DNA and RNA viruses. Microbiome9(1), 1-13. LINK
     
  • Roux, S., Páez-Espino, D., Chen, I. M. A., Palaniappan, K., Ratner, A., Chu, K., … & Kyrpides, N. C. (2021). IMG/VR v3: an integrated ecological and evolutionary framework for interrogating genomes of uncultivated viruses. Nucleic acids research49(D1), D764-D775. LINK
     
  • Tisza, M. J., & Buck, C. B. (2021). A catalog of tens of thousands of viruses from human metagenomes reveals hidden associations with chronic diseases. Proceedings of the National Academy of Sciences118(23).  LINK
     
  • Tisza, M. J., Belford, A. K., Dominguez-Huerta, G., Bolduc, B., & Buck, C. B. (2021). Cenote-Taker 2 democratizes virus discovery and sequence annotation. Virus evolution7(1), veaa100. LINK

Agenda

File

October 13 – Session 1: Virus Identification Tools

  • 1 pm Welcome & introduction – Matt Sullivan (Ohio State U., USA)
     
  • 1:30 pm VIBRANT – Karthik Anantharaman (U. of Wisconsin-Madison, USA)
     
  • 1:50 pm Cenote-Taker2 – Mike Tisza (NIAID-NIH, USA)
     
  • 2:10 pm VirSorter2 – Simon Roux (Joint Genome Institute, USA)
     
  • 2:30 – 3:30 pm Q&A and panel discussion

October 14 – Session 2: Virus Classification Tools

  • 1 pm Introduction – Evelien Adriaenssens (Quadram Institute, UK)
     
  • 1:30 pm VPF-Class – Joan Carles Pons (Balearic Islands U., Spain)
     
  • 1:50 pm VIRIDIC & VirClust – Cristina Moraru (U. of Oldenburg, Germany)
     
  • 2:10 pm vConTACT2 – Ben Bolduc (Ohio State U., USA)
     
  • 2:30 – 3:30 pm Q&A and panel discussion

October 15 – Session 3: Virus Databases

  • 1 pm Introduction – Joanne Emerson (U. of California, Davis, USA)
     
  • 1:15 pm IMG/VR – Simon Roux (Joint Genome Institute, USA)
     
  • 1:40 pm EBI and MGnify – Rob Finn (EBI)
     
  • 2:05 pm EVBC’s coming platform – Manja Marz (Friedrich Schiller U. Jena, Germany)
     
  • 2:30 – 3:30 pm Q&A and panel discussion

Speakers

Evelien Adriaenssens

Evelien Adriaenssens

Quadram Institute, UK

evelien adriaenssens

I am a molecular and computational microbiologist with a passion for everything viral.


I started my research career at the University of Leuven (KU Leuven, Belgium) investigating the use of bacteriophages (viruses of bacteria) as a biological control agent in potato plant production.


During my time as a postdoc (University of Pretoria, South Africa & University of Liverpool, UK), I specialised in viromics or viral metagenomics, to elucidate viral community diversity in a range of habitats, including hot and cold deserts, and the freshwater-marine continuum as impacted by wastewater.


I joined the Quadram Institute in January 2019 as a Career Track Group Leader, where my group is investigating the role of viruses, in particular bacteriophages, in the human gut.


We investigate how these viruses interact with bacteria, other microorganisms and the human host system. We aim to elucidate the role of viruses in the healthy gut across life.


We are also heavily involved in virus taxonomy, creating a genomic framework with which to understand global viral diversity.


We further aim to use newly isolated bacteriophages in the biocontrol of pathogenic bacteria in the fight against antimicrobial resistance and for the modulation of the microbiome to improve gut health.


I am the Chair of the Bacterial Viruses Subcommittee of the International Committee on Taxonomy of Viruses (ICTV).

Website: link | Google Scholar: link | Twitter: ​​@EvelienAdri
Workshop session: Virus classification

Sponsors

Ohio State University's Center of Microbiome Science
National Science Foundation
US Department of Energy